Professor John Colyer
BSc, CNAA; MPhil, London; PhD 1988, Southampton
Professor of Biotechnology
Institute of Membrane and Systems Biology
Background: BSc; CNAA; MPhil, London. PhD, Southampton. Post-doctoral work in the MRC Group in Signal Transduction, University of Calgary, Canada. British Heart Foundation Lecturer at University of Leeds, 1991-99. Senior Lecturer, 1999-current. Founder of Fluoroscience Ltd (biotech/drug discovery company), 1998
Office: Garstang 7.53b
Phone: +44(0) 113 34 33124
Email: j.colyer@leeds.ac.uk
Centre membership:
You can read more about Professor Colyer's interests here:
www.chem.leeds.ac.uk/SOMS/soms.html
www.bmb.leeds.ac.uk/staff/jc/homepage/homepage.htm
Research Interests
Natural & Therapeutic Control of Cardiac Function
The performance of many biological events is controlled through the transient chemical modification of components, or the partnering of new components in a cell. Where and when these events take place is key the process of control, and errors in these processes can lead to human disease. In my lab we are interested in the development of technologies which permit observation of these short-lived chemical events, and the application of these technologies to understand normal and abnormal cardiac performance.
The chemical adaptation of a small number of influential proteins changes the performance of the heart to meet the demands of exercise and stress, but this process fails following cardiac damage leading to life threatening loss of performance of the heart. We have developed the tools to examine these events, and are now capturing a mathematical description of the process of contraction and its control in health & disease (collaborators in Leeds, Vancouver & Auckland, NZ).
We are also developing novel experimental strategies for cardiac therapy. Having identified influential components in cardiac biology, we have engineered a strategy in which the diseased component (a protein) is removed by molecular intervention and replaced with a designer version of the component which corrects the malfunction. This strategy is being developed with a cardiac protein component, but has applications across medicine & biotechnology. Current funding is from BHF, EPSRC and an UK Biotechnology Co.
Studentship information
Undergraduate project topics:
- Protein degradation control as a therapeutic strategy
Keywords: Heterologous expression, yeast, pulse-chase, kinetics. (Laboratory) -
Protein phosphorylation in cardiac muscle contraction
Keywords: Signal transduction, cardiac myocytes, immunotechniques (blotting/microscopy), microinjection (Laboratory) -
Control of cellular differentiation: Cellular transplantation therapies
Keywords: In title (Literature)
Postgraduate studentship areas:
- Biological engineering and Cell biology of Cardiac muscle Phosphoproteins
See also:
- Faculty Graduate School
- Project details at FindaPhD.com
Publications
MacDougall DA; Agarwal SR; Stopford EA; Chu H; Collins JA; Longster AL; Colyer J; Harvey RD; Calaghan S Caveolae compartmentalise beta 2-adrenoceptor signals by curtailing cAMP production and maintaining phosphatase activity in the sarcoplasmic reticulum of the adult ventricular myocyte JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY 52 388-400, 2012
DOI:10.1016/j.yjmcc.2011.06.014
Carter S; Pitt SJ; Colyer J; Sitsapesan R Ca2+-Dependent Phosphorylation of RyR2 Can Uncouple Channel Gating from Direct Cytosolic Ca2+ Regulation JOURNAL OF MEMBRANE BIOLOGY 240 21-33, 2011
DOI:10.1007/s00232-011-9339-9
Chase A; Colyer J; Orchard CH Localised Ca channel phosphorylation modulates the distribution of L-type Ca current in cardiac myocytes JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY 49 121-131, 2010
DOI:10.1016/j.yjmcc.2010.02.017
Johnson BRG; Barfoot R; Sheikh K; Colyer J; Bushby RJ; Evans SD COLL 56-Actin scaffolds on supported and suspended bilayer lipid membranes, 2008
Barfoot RJ; Sheikh KH; Johnson BRG; Colyer J; Miles RE; Jeuken LJC; Bushby RJ; Evans SD Minimal F-actin cytoskeletal system for planar supported phospholipid bilayers LANGMUIR 24 6827-6836, 2008
DOI:10.1021/la800085n
Locate S; Colyer J; Gawler DJ; Walker JH Annexin A6 at the cardiac myocyte sarcolemma - Evidence for self-association and binding to actin CELL BIOLOGY INTERNATIONAL 32 1388-1396, 2008
DOI:10.1016/j.cellbi.2008.08.009
Carter S; Colyer J; Sitsapesan R Maximum phosphorylation of the cardiac ryanodine receptor at serine-2809 by protein kinase A produces unique modifications to channel gating and conductance not observed at lower levels of phosphorylation CIRCULATION RESEARCH 98 1506-1513, 2006
DOI:10.1161/01.RES.0000227506.43292.df
Johnson BRG; Bushby RJ; Colyer J; Evans SD Assembly of actin scaffolds at ponticulin-containing supported phospholipid bilayers BIOPHYSICAL JOURNAL 90 L21-L23, 2006
DOI:10.1529/biophysj.105.076521
Jones PP; Bazzazi H; Kargacin GJ; Colyer J Inhibition of cAMP-dependent protein kinase under conditions occurring in the cardiac dyad during a Ca2+ transient BIOPHYSICAL JOURNAL 91 433-443, 2006
DOI:10.1529/biophysj.106.083931
Johnson BRG; Sheikh K; Evans SD; Colyer J; Bushby RJ Hierarchical self assembly of cytoskeletal scaffolds bound to supported phospholipid bilayers., 2005
Evans SD; Johnson BRG; Bushby RJ; Colyer J; Jeuken L; Miles R Membranes, scaffolds and surfaces., 2005
Brette F; Rodriguez P; Komukai K; Colyer J; Orchard CH beta-adrenergic stimulation restores the Ca transient of ventricular myocytes lacking t-tubules JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY 36 265-275, 2004
DOI:10.1016/j.yjmcc.2003.11.002
Rodriguez P; Jackson WA; Colyer J Critical evaluation of cardiac Ca2+-ATPase phosphorylation on serine 38 using a phosphorylation site-specific antibody JOURNAL OF BIOLOGICAL CHEMISTRY 279 17111-17119, 2004
DOI:10.1074/jbc.M400462200
Bhogal MS; Colyer J Phospholamban degradation: Routes and rates of degradation in model eukaryotic, 2003
Sarojini VSA; Bacchus PS; Colyer J Template assembled biosensor based on M2 ion channel Biophysical Journal 84 pp.134-, 2003
Sarojini V; Bacchus P; Colyer J Template assembled-biosensor based on the M2 ion channel, 2003
Rodriguez P; Bhogal MS; Colyer J RYR2 phosphorylation: can PKA phosphorylate the ryanodine receptor at Ser-2809? Biophysical Journal 84 pp.99-, 2003
Rodriguez P; Bhogal MS; Colyer J RyR2 phosphorylation: can PKA phosphorylate the Ryanodine receptor at Ser-2809?, 2003
Bhogal MS; Colyer J Phospholamban degradation: Routes and rates of degradation in model eukaryotic cells Biophysical Journal 84 pp.101-, 2003
Jones PP; Colyer J Identification of state of filling kinase from cardiac SR Biophysical Journal 84 pp.99-, 2003
Jones PP; Colyer J Identification of state of filling kinase from cardiac SR., 2003
Rodriguez P; Bhogal MS; Colyer J Stoichiometric phosphorylation of cardiac ryanodine receptor on serine 2809 by calmodulin-dependent kinase II and protein kinase A JOURNAL OF BIOLOGICAL CHEMISTRY 278 38593-38600, 2003
DOI:10.1074/jbc.C301180200
Ng YL; Goldspink DF; Burniston JG; Clark WA; Colyer J; Tan LB Characterisation of isoprenaline myotoxicity on slow-twitch skeletal versus cardiac muscle INTERNATIONAL JOURNAL OF CARDIOLOGY 86 299-309, 2002
DOI:10.1016/S0167-5273(02)00369-8
Goldspink D; Burniston J; Cox H; Clark W; Colyer J; Tan LB Catecholamine-induced myocyte necrosis in cardiac and skeletal muscles of the rat, 2002
Brette F; Rodriguez P; Colyer J; Orchard CH Effect of detubulation on phospholamban phosphorylation in rat ventricular myocytes, 2002
Brette FP; Rodriguez P; Colyer J; Orchard CH Effect of detubulation on phospholamban phosphorylation in rat ventricular myocytes Journal of Physiology 544 46-46, 2002
Burniston JG; Ng Y; Clark WA; Colyer J; Tan LB; Goldspink DF Myotoxic effects of clenbuterol in the rat heart and soleus muscle JOURNAL OF APPLIED PHYSIOLOGY 93 1824-1832, 2002
DOI:10.1152/japplphysiol.00139.2002
Aly A; Hunter M; Knowles PF; Colyer J Phospholamban exhibits regulated ion channel activity BIOPHYSICAL JOURNAL 80 26A-26A, 2001
Locate S; Adegunloye BJ; Colyer J; Walker JH Annexin VI binds to calsequestrin of skeletal muscle sarcoplasmic reticulum FASEB JOURNAL 15 A816-A816, 2001
Locate S; Adegunloye BJ; Colyer J; Walker JH Annexin VI binds to calsequestrin of skeletal muscle sarcoplasmic reticulum The FASEB Journal 15 816-816, 2001
Bhogal MS; Colyer J The rate of phospholamban degradation in cultured cardiac myocytes BIOPHYSICAL JOURNAL 80 586A-587A, 2001
Brette F; Calaghan SC; Lappin S; White E; Colyer J; Le Guennec JY Biphasic effects of hyposmotic challenge on excitation-contraction coupling in rat ventricular myocytes AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY 279 H1963-H1971, 2000
McDonald RL; Colyer J; Harrison SM Quantitative analysis of Na+-Ca2+ exchanger expression in guinea-pig heart EUROPEAN JOURNAL OF BIOCHEMISTRY 267 5142-5148, 2000
DOI:10.1046/j.1432-1327.2000.01579.x
Calaghan SC; Colyer J; White E Cyclic AMP but not phosphorylation of phospholamban contributes to the slow inotropic response to stretch in ferret papillary muscle PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 437 780-782, 1999
DOI:10.1007/s004240050846
Levine BA; Patchell VB; Sharma P; Gao Y; Bigelow DJ; Yao Q; Goh S; Colyer J; Drago GA; Perry SV Sites on the cytoplasmic region of phospholamban involved in interaction with the calcium-activated ATPase of the sarcoplasmic reticulum EUROPEAN JOURNAL OF BIOCHEMISTRY 264 905-913, 1999
DOI:10.1046/j.1432-1327.1999.00688.x
McDonald RL; Colyer J; Harrison SM Regional variations in the location of Na-Ca exchanger protein in guinea-pig heart at cellular and tissue levels BRITISH JOURNAL OF PHARMACOLOGY 128 U19-U19, 1999
Hussain M; Drago GA; Bhogal M; Colyer J; Orchard CH Effects of the protein kinase A inhibitor H-89 on Ca2+ regulation in isolated ferret ventricular myocytes PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 437 529-537, 1999
DOI:10.1007/s004240050814
McDonald RL; Drago GA; Colyer J; Harrison SM Expression of Na+-Ca2+ exchanger in guinea-pig left ventricle BIOPHYSICAL JOURNAL 76 A305-A305, 1999
Palfreyman T; Hussain M; Drago GA; Berrebi-Bertrand I; Bril A; Orchard CH; Colyer J The importance of protein phosphatase 1 in cardiac CA(2+) homeostasis BIOPHYSICAL JOURNAL 74 A379-A379, 1998
Calaghan SC; Colyer J; White E Co-ordinated changes in cAMP, phosphorylated phospholamban, Ca2+ and contraction following beta-adrenergic stimulation in isolated rat ventricular myocytes JOURNAL OF PHYSIOLOGY-LONDON 509P 142P-143P, 1998
Calaghan SC; Colyer J; White E Length-dependent modulation of cAMP and the phosphorylation of phospholamban in isolated ferret papillary muscle JOURNAL OF PHYSIOLOGY-LONDON 511P 82P-83P, 1998
Ng Y; Colyer J; Tan LB; Clark WA; Goldspink DF Rat striated muscles damaged by isoprenaline JOURNAL OF PHYSIOLOGY-LONDON 511P 157P-158P, 1998
Drago GA; Colyer J; Lederer WJ Immunofluorescence localization of SERCA2a and the phosphorylated forms of phospholamban in intact rat cardiac ventricular myocytes CARDIAC SARCOPLASMIC RETICULUM FUNCTION AND REGULATION OF CONTRACTILITY 853 273-279, 1998
DOI:10.1111/j.1749-6632.1998.tb08278.x
Bhogal MS; Colyer J Depletion of sarcoplasmic reticulum calcium prompts phosphorylation of phospholamban to stimulate store refilling CARDIAC SARCOPLASMIC RETICULUM FUNCTION AND REGULATION OF CONTRACTILITY 853 260-263, 1998
DOI:10.1111/j.1749-6632.1998.tb08274.x
Colyer J Phosphorylation states of phospholamban CARDIAC SARCOPLASMIC RETICULUM FUNCTION AND REGULATION OF CONTRACTILITY 853 79-91, 1998
DOI:10.1111/j.1749-6632.1998.tb08258.x
Colyer J Chemical Modification, 1998
Calaghan SC; White E; Colyer J Co-ordinated changes in cAMP, phosphorylated phospholamban, Ca2+ and contraction following beta-adrenergic stimulation of rat heart PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 436 948-956, 1998
DOI:10.1007/s004240050728
Bhogal MS; Colyer J Depletion of Ca2+ from the sarcoplasmic reticulum of cardiac muscle prompts phosphorylation of phospholamban to stimulate store refilling PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 95 1484-1489, 1998
DOI:10.1073/pnas.95.4.1484
Calaghan S; White E; Colyer J Preservation of the in vivo phosphorylation status of phospholamban in the heart: Evidence for a site-specific difference in the dephosphorylation of phospholamban BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 248 701-705, 1998
DOI:10.1006/bbrc.1998.9036
Ng Y; Wright H; Colyer J; Clark WA; Tan LB; Goldspink DF Localization of isoprenaline induced damage in myocytes of striated muscle. JOURNAL OF PHYSIOLOGY-LONDON 504P P74-P74, 1997
Hulme JT; Colyer J; Orchard CH Acidosis alters the phosphorylation of Ser(16) and Thr(17) of phospholamban in rat cardiac muscle PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 434 475-483, 1997
DOI:10.1007/s004240050423
Hussain M; Drago GA; Colyer J; Orchard CH Rate-dependent abbreviation of Ca2+ transient in rat heart is independent of phospholamban phosphorylation American Journal of Physiology - Heart and Circulatory Physiology 273 H695-H706, 1997
Hussain M; Drago GA; Colyer J; Orchard CH Rate-dependent abbreviation of Ca2+ transient in rat heart is independent of phospholamban phosphorylation. Am J Physiol 273 H695-H706, 1997
Hussain M; Orchard CH; Colyer J Effects of the protein kinase A (PKA) inhibitor H89 on the Ca2+ current (I-Ca) and the Ca2+ transient in isolated ferret ventricular myocytes. JOURNAL OF PHYSIOLOGY-LONDON 504P P79-P80, 1997
Hussain M; Drago GA; Colyer J; Orchard CH Rate-dependent abbreviation of Ca2+ transient in rat heart is independent of phospholamban phosphorylation AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY 273 H695-H706, 1997
Jackson WA; Colyer J Translation of Ser(16) and Thr(17) phosphorylation of phospholamban into Ca2+-pump stimulation BIOCHEMICAL JOURNAL 316 201-207, 1996
Calaghan SC; White E; Colyer J Quantitative analysis of the phosphorylation of phospholamban in isolated ferret papillary muscle JOURNAL OF PHYSIOLOGY-LONDON 497P P3-P4, 1996
Hussain M; Drago GA; Colyer J; Orchard CH Inhibitors of Ca2+-calmodulin-dependent protein kinase II (CaM kinase II) do not prevent rate-dependent abbreviation of the Ca2+ transient in isolated rat ventricular myocytes JOURNAL OF PHYSIOLOGY-LONDON 497P P49-P49, 1996
Drago GA; Hussain M; Colyer J Temporal resolution of phospholamban phosphorylation and dephosphorylation in vivo FASEB JOURNAL 10 1810-1810, 1996
Quirk PG; Patchell VB; Colyer J; Drago GA; Gao Y Conformational effects of serine phosphorylation in phospholamban peptides EUROPEAN JOURNAL OF BIOCHEMISTRY 236 85-91, 1996
DOI:10.1111/j.1432-1033.1996.00085.x
BENNETT K; COLYER J; ORCHARD C THE EFFECT OF STIMULATION RATE AND BATHING [CA2+] ON THE PHOSPHORYLATION OF PHOSPHOLAMBAN IN ISOLATED RAT VENTRICULAR MYOCYTES JOURNAL OF PHYSIOLOGY-LONDON 483P P20-P20, 1995
YANG Z; BOYETT M; JANVIER N; HULME J; ORCHARD C; COLYER J DIFFERENCES IN THE TIME-COURSE OF CONTRACTION IN SUB-EPICARDIAL AND SUB-ENDOCARDIAL CELLS ISOLATED FROM THE LEFT-VENTRICLE OF THE DOG AND FERRET JOURNAL OF PHYSIOLOGY-LONDON 487P P127-P127, 1995
HULME J; COLYER J; ORCHARD C THE EFFECTS OF ACIDOSIS ON THE PHOSPHORYLATION OF SER(16) AND THR(17) OF PHOSPHOLAMBAN IN RAT CARDIAC-MUSCLE JOURNAL OF PHYSIOLOGY-LONDON 487P P128-P129, 1995
BENNETT K; WHITE E; COLYER J THE EFFECT OF CHANGES IN MECHANICAL LOADING ON THE PHOSPHORYLATION OF PHOSPHOLAMBAN IN ISOLATED FERRET PAPILLARY-MUSCLE JOURNAL OF PHYSIOLOGY-LONDON 487P P141-P141, 1995
DRAGO G; COLYER J PHOSPHORYLATION SITE-SPECIFIC ANTIBODIES TO CARDIAC PHOSPHOLAMBAN, 1995
SMITH F; COLYER J EXPRESSION AND PURIFICATION OF CARDIAC PHOSPHOLAMBAN IN ESCHERICHIA-COLI, 1995
Drago GA; Colyer J Phosphorylation site-specific antibodies to cardiac phospholamban Biochemical Society Transactions 23 -, 1995
Smith FK; Colyer J Expression and purification of cardiac phospholamban in Escherichia coli Biochemical Society Transactions 23 -, 1995
Jackson WA; Colyer J Release of Ca2+-Mg2+ ATPase inhibition by phospholamban phosphorylation Biochemical Society Transactions 23 -, 1995
DRAGO G; COLYER J DISCRIMINATION BETWEEN 2 SITES OF PHOSPHORYLATION ON ADJACENT AMINO-ACIDS BY PHOSPHORYLATION SITE-SPECIFIC ANTIBODIES TO PHOSPHOLAMBAN JOURNAL OF BIOLOGICAL CHEMISTRY 269 25073-25077, 1994
COLYER J CONTROL OF THE CALCIUM-PUMP OF CARDIAC SARCOPLASMIC-RETICULUM - A SPECIFIC ROLE FOR THE PENTAMERIC STRUCTURE OF PHOSPHOLAMBAN CARDIOVASCULAR RESEARCH 27 1766-1771, 1993
DOI:10.1093/cvr/27.10.1766
MORRIS G; CHENG H; COLYER J; WANG J PHOSPHOLAMBAN REGULATION OF CARDIAC SARCOPLASMIC-RETICULUM (CA2+-MG2+)-ATPASE - MECHANISM OF REGULATION AND SITE OF MONOCLONAL-ANTIBODY INTERACTION JOURNAL OF BIOLOGICAL CHEMISTRY 266 11270-11275, 1991
Mata AM; Colyer J; Michelangeli F; Lee AG; East JM The effect of a monoclonal antibody on specific steps of the reaction sequence of the (Ca2+-Mg2+)-ATPase from sarcoplasmic reticulum Biochemical Society Transactions 19 -, 1991
COLYER J; WANG J DEPENDENCE OF CARDIAC SARCOPLASMIC-RETICULUM CALCIUM-PUMP ACTIVITY ON THE PHOSPHORYLATION STATUS OF PHOSPHOLAMBAN JOURNAL OF BIOLOGICAL CHEMISTRY 266 17486-17493, 1991
MICHELANGELI F; COLYER J; EAST J; LEE A EFFECT OF PH ON THE ACTIVITY OF THE CA-2++MG-2+-ACTIVATED ATPASE OF SARCOPLASMIC-RETICULUM BIOCHEMICAL JOURNAL 267 423-429, 1990
LI C; WANG J; COLYER J IMMUNOLOGICAL DETECTION OF PHOSPHOLAMBAN PHOSPHORYLATION STATES FACILITATES THE DESCRIPTION OF THE MECHANISM OF PHOSPHORYLATION AND DEPHOSPHORYLATION BIOCHEMISTRY 29 4535-4540, 1990
DOI:10.1021/bi00471a005
MOVSESIAN M; LEVEILLE C; KRALL J; COLYER J; WANG J; CAMPBELL K IDENTIFICATION AND CHARACTERIZATION OF PROTEINS IN SARCOPLASMIC-RETICULUM FROM NORMAL AND FAILING HUMAN LEFT-VENTRICLES JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY 22 1477-1485, 1990
DOI:10.1016/0022-2828(90)90990-J
BURGOYNE R; CHEEK T; MORGAN A; OSULLIVAN A; MORETON R; BERRIDGE M; MATA A; COLYER J; LEE A; EAST J DISTRIBUTION OF 2 DISTINCT CA-2+-ATPASE-LIKE PROTEINS AND THEIR RELATIONSHIPS TO THE AGONIST-SENSITIVE CALCIUM STORE IN ADRENAL CHROMAFFIN CELLS NATURE 342 72-74, 1989
DOI:10.1038/342072a0
COLYER J; MATA A; LEE A; EAST J EFFECTS ON ATPASE ACTIVITY OF MONOCLONAL-ANTIBODIES RAISED AGAINST (CA-2+ + MG-2+)-ATPASE FROM RABBIT SKELETAL-MUSCLE SARCOPLASMIC-RETICULUM AND THEIR CORRELATION WITH EPITOPE LOCATION BIOCHEMICAL JOURNAL 262 439-447, 1989
MATTHEWS I; COLYER J; MATA A; GREEN N; SHARMA R; LEE A; EAST J EVIDENCE FOR THE CYTOPLASMIC LOCATION OF THE N-TERMINAL AND C-TERMINAL SEGMENTS OF SARCOPLASMIC-RETICULUM (CA2+-MG2+)-ATPASE BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 161 683-688, 1989
DOI:10.1016/0006-291X(89)92653-3
KUMAR P; WALKERSMITH J; MILLA P; HARRIS G; COLYER J; HALLIDAY R THE TEENAGE CELIAC - FOLLOW UP STUDY OF 102 PATIENTS ARCHIVES OF DISEASE IN CHILDHOOD 63 916-920, 1988
COLYER J; KUMAR P; WALDRON N; CLARK M; FARTHING M GLIADIN BINDING TO RAT AND HUMAN ENTEROCYTES CLINICAL SCIENCE 72 593-598, 1987
COLYER J; FARTHING M; KUMAR P; CLARK M; OHANNESIAN A; WALDRON N REAPPRAISAL OF THE LECTIN HYPOTHESIS IN THE ETIOPATHOGENESIS OF CELIAC-DISEASE CLINICAL SCIENCE 71 105-110, 1986
